Indicate which reference genome E-CRISP should find targets in?
Indices are pre-built for each organism's individual gene sequences
Enter either:

a ";" separated list of official gene symbols or Accession IDs. For e.g.

PFKP;PFKFB3;PRKCQ;CAMK1D (for Human)
ENSDARG00000090752;ENSDARG00000063570;ENSDARG00000074914;ENSDARG00000032801 (for Zebrafish)
FBgn0000442;FBgn0250906;FBgn0020618;FBgn0044323 (for Fly)


OR

one or more Fasta formatted sequence. For e.g:

>sequenceID
ATATCGATGCTAGCTAGCT
GATCGTAGCTAGCTAGCTA

Transcript IDs (ENST...) can be entered in subsection 4
but still require the respective gene to be entered here.
Fasta file format.

>sequenceID
ATATCGATGCTAGCTAGCT
GATCGTAGCTAGCTAGCTA

OR:

a file containing a list of official ENSEMBL gene symbols and/or Accession IDs, one gene per line.
For e.g.

HumanZebrafishFly
ENSG00000067057ENSDARG00000090752FBgn0000442
ENSG00000170525ENSDARG00000063570FBgn0250906
ENSG00000065675ENSDARG00000074914FBgn0020618
ENSG00000183049ENSDARG00000032801FBgn0044323


Maximum file size: 20 MB.
Minimum length of the nucleotide sequence one arm of the CRISPR dimer should recognize.
Maximum length of the nucleotide sequence one arm of the CRISPR dimer should recognize.
Adjust the length of the sequence that will be saved 3' to the cut site.
Adjust the length of the sequence that will be saved 5' to the cut site.
Check if designs should only target genes.
Check if designs should only target exons.
Check if designs should target only outside of predicted CpG islands.
(Outside potential hypermethylated regions)
Enter a specific ensembl transcript ID ENSTR.......
Enter the exon number to target. E.g. Enter 1 to find designs which target the first exon exon.
Or enter the word any to find designs that hit any exon.
Check this box to assess restricion enzyme cut sites in the whole target sequence.
Choose between different pre-defined restriction enzyme subsets.
Leave this box checked if designs should be analysed for targets in the reference genome.
Should the CRISPR design be checked for any secondary off-target effects?
This is useful to check if they will cut in any exogenous, foreign introduced sequence.
Check every individual off-target or paste a set of fasta sequences into the text area
This text area only accepts FASTA format sequences as input e.g.:

>some sequence
ATATCGATGCTAGCTAGCT
GATCGTAGCTAGCTAGCTA
E-CRISP
Design of CRISPR constructs
DKFZ
De-novo Evaluation Help Links

1. Select organism:

[HELP]


2. Select target region by gene symbol or sequence:

Enter Target Sequence
Clear

FASTA example [HELP]
Search by gene symbol

Search results (click to add, scroll down to see full list)

    Search results (click to remove, scroll down to see full list)


      3. Start application:

      relaxed
      (any PAM (NAG/NGG), any 5' base (A,C,G,T), off-targets need full length perfect match, introns are allowed)

      medium
      (any PAM (NAG/NGG), any 5' base (A,C,G,T), off-targets tolerate mismatches, introns/CPG islands are excluded)

      strict
      (only NGG PAM, only G as 5' base, off-target tolerates many mismatches and ignores non-seed region, introns,CPG islands and UTRs are excluded)




      The older version of E-CRISP can be reached Here




      Boutros lab, E-CRISP-Version 4.1
      For suggestions please contact us at crispr@dkfz.de